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KMID : 0357920020360020077
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Korean Journal of Pathology
2002 Volume.36 No. 2 p.77 ~ p.83
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Ras Gene Mutations and Expression of ERK1 and ERK2 Proteins in Stomach Cancer
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Yoo Jin-Young
Kang Chang-Suk
Kang Seok-Jin
Kim Byung-Kee
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Abstract
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Background: We investigated stomach cancers for ras abnormalities and expression of ERK1 and ERK2 to determine their significance in the tumor development and/or progression and to evaluate their potential correlation with clinicopathologic parameters.
Methods: Seventy gastric adenocarcinomas were studied immunohistochemically in paraffin-embedded tissue sections for the expression of ERK1 and ERK2 proteins. All tumors were further analyzed with the use of a polymerase chain reaction technique and a direct sequence analysis procedure for the presence of the mutated ras gene.
Results: ERK1 and/or ERK2 was expressed in 65.7%(46/70) of the tumors; overexpression of ERK1 was observed in 38 (54.3%) tumors, whereas ERK2 was detected in 29 (41.4%). Nine (12.8%) samples demonstrated mutations in the rs gene: 4 in H-ras and 5 in K-ras. Seven of the 9(77.8%) mutated tumors were of the intestinal type. No assocition was established between the ras abnormatilites and the overexpression of ERK1 and/or ERK2. However, the correlation between ERK2 and progressio (early vs. advanced) was statistically significant (p<0.05).
Conclusions: These data indicate that ras abnormalities are uncommon events in gatstric adenocarcinomas. The majority of ras mutations, however, occurred in intestinal-type tumors, supporting the notion of different molecular mechanisms involved between the intestinal- and diffuse-type lesions. Enhanced ERK2 activity may provide assistance in the determinatino of tumor penetration in these tumors.
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KEYWORD
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Stomach Neoplasms-Genes, ras-MAP Kinase Signaling System
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